Toxicity Test for the Extract of Symbiont Bacteria Bacillus sp. as Anti-bacteria

Increased antibiotic resistance spurs exploration of bioactive compounds as new antibiotic alternatives. Bacillus sp. is a symbiont bacterium which is a marine microorganism that has the potential to produce new bioactive compounds that can be developed as new antibiotics. This research is an experimental study aimed at identifying bioactive compounds by thin layer chromatography methods and testing the activity of bioactive compounds by probit analysis EPA probit analysis program version 1.5. in Artemia salina Leach. Bioactive compounds identified were compounded from the alkaloid group with the category of highly toxic several-irritating base on the EPA toxicity category and the highly hazardous base on The WHO toxicity category based on the environmental protection agency probit analysis program used for calculating LC / EC value of LC 50 = 169,520 g / l. Symbiont Bacillus sp. produce secondary metabolites in the form of bioactive which have the ability as anti-bacteria. As a new antibiotic alternative to overcome resistance, especially in methicillin-resistant bacteri

PEMBUATAN APLIKASI BAHAYA ROKOK BERBASIS ANDROID

An understanding of the dangers of cigarettes in Indonesian society has beenwidely carried out, both through online and offline, but often less effectivebecause the media used is less attractive and complicated in its use. This studyaims to make the media practical, interesting and easy to use by utilizing theandroid application of the dangers of mobile-based smoking online. Thisapplication is expected to be one of the interesting and easy ways to use to helpunderstand the dangers of smoking so that people can easily access thisapplication. Android-based mobile application uses the app inventor method,this application contains the notion of cigarettes, smoking habits and the dangersof smoking and the meaning of words and warning warnings contained incigarette packs. In this application, there are text, images, videos about thedangers of smoking, clinics stop smoking and videos about the dangers ofsmoking. Android-based applications are presented with easy to understand andapplicable, so this application can simplify and provide an explanation of smokingfor mobile phone users and it is expected that users who have a smoking habitwill realize and reduce and even stop smoking.Keywords: Media online, mobile phone, the dangers of smoking, android basedapplications, reduce smoking habit

PEMBUATAN APLIKASI BAHAYA ROKOK BERBASIS ANDROID

An understanding of the dangers of cigarettes in Indonesian society has beenwidely carried out, both through online and offline, but often less effectivebecause the media used is less attractive and complicated in its use. This studyaims to make the media practical, interesting and easy to use by utilizing theandroid application of the dangers of mobile-based smoking online. Thisapplication is expected to be one of the interesting and easy ways to use to helpunderstand the dangers of smoking so that people can easily access thisapplication. Android-based mobile application uses the app inventor method,this application contains the notion of cigarettes, smoking habits and the dangersof smoking and the meaning of words and warning warnings contained incigarette packs. In this application, there are text, images, videos about thedangers of smoking, clinics stop smoking and videos about the dangers ofsmoking. Android-based applications are presented with easy to understand andapplicable, so this application can simplify and provide an explanation of smokingfor mobile phone users and it is expected that users who have a smoking habitwill realize and reduce and even stop smoking.Keywords: Media online, mobile phone, the dangers of smoking, android basedapplications, reduce smoking habit

Development of an Evidence-Based Tool to Assess the Relative Vulnerability of Different Communities to Tuberculosis

Identifying specific tuberculosis (TB) vulnerabilities in populations based on their geographical, demographic, and epidemiological characteristics is an essential yet challenging requirement to help reduce and eliminate TB. Assessment tools that can accurately quantify the risks associated with key factors could be used to measure TB vulnerability efficiently and indicate the most appropriate range of interventions. This study aimed to develop TB vulnerability assessment tools based on a TB vulnerability assessment conceptual framework developed with Leximancer. Three steps to produce the tools were facet analysis, interpreting the facet to create a list of questions, and expert judgment to confirm the suitability of the questionnaire. The “everything is data” principle was used to identify the data sources and build the tools. The data came from multiple primary data sources, with a questionnaire survey and observational form, and secondary data from various governmental statistical departments in Indonesia to collect data related to demography, health indicators, climate, temperature, and air quality. These tools will be optimized at scale next year to evaluate their utility for prioritizing and prescribing health system responses to TB in different communities in Central Java Province

BIOREMEDIASI PESTISIDA GOLONGAN ORGANOPHOSPHAT (MALATHION DAN PROFENOFOS) OLEH BAKTERI INDIGENOUS TERSELEKSI DARI PERAIRAN RAWA PENING. KABUPATEN SEMARANG

Rawa Pening merupakan suatu sistem ekologis perairan air tawar berbentuk danau yang di dalamnya terjadi suatu hubungan interaksi antara faktor biotik, abiotik dan dekomposer yang membentuk suatu keseimbangan. Hadirnya pestisida pada perairan Rawa Pening dapat menyebabkan terganggunya keseimbangan ekologi yang menyebabkan terjadinya pencemaran dan jika berlangsung dalam jangka waktu lama dapat menyebabkan bioakumulasi pada ekosistem Rawa Pening. Bioremediasi Pestisida Organophosphat (Profenofos dan Malathion) dengan memanfaatkan bakteri Indigenous dari Rawa Pening adalah suatu upaya mengembalikan kesimbangan ekologis parairan akibat pencemaran Profenofos dan Malathion. Pada Proses Bioremediasi diperlukan bakteri terpilih, terseleksi oleh karena itu diperlukan proses skrining yang meliputi langkah isolasi, seleksi dan identifikasi. Proses Skrining dilakukan secara kualitatif dan kuatitatif dengan metode eksperimental di laboratorium. 1. Uji kualititatif dilakukan dengan mengamati perubahan warna pada substrat Malathion dan Profenofos dengan Bromtymol Blue sebagai indikator perubahan warna. 2. Uji kuantitatif dengan metode Spektofotmetry dan Gas Chromatography Mass Spectrofotometry (GC-MS). 3. Berdasarkan seleksi bakteri melalui uji kualitatif dan kuantitatif adalah tiga bakteri terbaik dengan kode RPL-5, TRA-5 dan RPL-1. Bakteri dengan kode TRA-5 memiliki kemampuan degradasi terbaik pada substrat Profenofos dan bakteri dengan kode RPL-5 memilki kemampuan degrdasi terbaik pada susbtrat Malathion. Bakteri terbaik selanjutnya di uji sinergisitasnya untuk mendapatkan bakteri konsorsium terbaik yang akan digunakan dalam uji degradasi pada skala aplikasi terbatas pada air dan sedimen Rawa Pening pada konsetrasi maksimum, sebagai berikut : a) Bakteri konsorsium RPL-1 dan RPL-5 memilki kemampuan degradasi terbaik pada air Rawa Pening dengan konsentrasi Malathion b) Bakteri konsorsium RPL-5 dan TRA-5 memiliki kemampuan degradasi terbaik pada air Rawa Pening dengan konsentrasi Profenofos. Lake Rawa Pening is an ecological system which happens to a relationship the interaction between biotic factors, abiotic factors and microbial organisms, which establish a balance in it. The presence of pesticides in Rawa Pening waters led to disruption of ecological balance causing the occurrence of pollution and if it takes place in a prolonged period of time can lead to bio-accumulation at ecosystem Rawa Pening Bioremediation of pesticide Organophosphat (Profenofos and Malathion) and utilize Indigenous bacteria from Rawa Pening is an effort to restore the ecologica balance of the water due to leaching of Profenofos and Malathion. In the process of Bioremediation bacteria required elected and selected therefore required a screening process involves the isolation, identification and genetic information to get the bacteria have the ability best degradation. Screening is done by qualitative and quantitative experimental method in the laboratory. 1) Qualitative Test is done by observing the color change with Bromtymol Blue as an indicator changes color on solid zobell media enriched with Malathion and Profenofos.). 2) Quantitative tests done by the method of Gas Chromatography and Mass Spektofotmetry Spectrofotometry (GC-MS). 3) Based on the selection of bacteria through qualitative and quantitative testing are the three best bacteria with code RPL -5, TRA-5 and RPL-1. Bacteria with the code TRA-5 has the best substrate degradation abilities of Profenofos and bacteria with code RPL-5 has the best degradation on susbtrat ability of Malathion The next best in the bacterial synergistic test to get the best Consortium bacteria to be used in a test of degradation on a scale of application is limited to water and sediment Rawa Pening on maximum concentrations, as follows : 1) Bacterial Consortium with the code RPL-5 and RPL-1 has the best on subastrat degradation ability test of water from Rawa Pening with the addition of concentrations of Malathion 2) Bacterial Consortium with the code RPL-5 and TRA-5 has the best on subastrat degradation ability test of water from Rawa Pening with the addition of concentrations of Profenofos xlii Identification of bacteria selected and most superior in Malathion degrades and Profenofos done in molecular biology shows the following results: 1) The maximum identity similarity levels of bacteria RPL-1 is 87% and based on the analysis of the phylogenetic tree, the bacteria isolates RPL-1 had the closest kinship with Oceanobacillus iheyenis 2) The maximum identity similarity levels of bacteria RPL-5 is 99% and based on the analysis of the phylogenetic tree, the bacteria isolates RPL-5 has the closest kinship with the bacteria Exiquobacterium profundum 3) The maximum identity similarity levels of TRA-5 is 98% based on the phylogenetic tree analysis of bacterial isolates, TRA-5 has a very close kinship with Bacillus firmus Keywords: Pollution Of Pesticide, Malathion, Profenofos, Bioremediation, Molecular Biology Degradation, Bioremediatio

BIOREMEDIASI PESTISIDA GOLONGAN ORGANOPHOSPHAT (MALATHION DAN PROFENOFOS) OLEH BAKTERI INDIGENOUS TERSELEKSI DARI PERAIRAN RAWA PENING. KABUPATEN SEMARANG

Rawa Pening merupakan suatu sistem ekologis perairan air tawar berbentuk danau yang di dalamnya terjadi suatu hubungan interaksi antara faktor biotik, abiotik dan dekomposer yang membentuk suatu keseimbangan. Hadirnya pestisida pada perairan Rawa Pening dapat menyebabkan terganggunya keseimbangan ekologi yang menyebabkan terjadinya pencemaran dan jika berlangsung dalam jangka waktu lama dapat menyebabkan bioakumulasi pada ekosistem Rawa Pening. Bioremediasi Pestisida Organophosphat (Profenofos dan Malathion) dengan memanfaatkan bakteri Indigenous dari Rawa Pening adalah suatu upaya mengembalikan kesimbangan ekologis parairan akibat pencemaran Profenofos dan Malathion. Pada Proses Bioremediasi diperlukan bakteri terpilih, terseleksi oleh karena itu diperlukan proses skrining yang meliputi langkah isolasi, seleksi dan identifikasi. Proses Skrining dilakukan secara kualitatif dan kuatitatif dengan metode eksperimental di laboratorium. 1. Uji kualititatif dilakukan dengan mengamati perubahan warna pada substrat Malathion dan Profenofos dengan Bromtymol Blue sebagai indikator perubahan warna. 2. Uji kuantitatif dengan metode Spektofotmetry dan Gas Chromatography Mass Spectrofotometry (GC-MS). 3. Berdasarkan seleksi bakteri melalui uji kualitatif dan kuantitatif adalah tiga bakteri terbaik dengan kode RPL-5, TRA-5 dan RPL-1. Bakteri dengan kode TRA-5 memiliki kemampuan degradasi terbaik pada substrat Profenofos dan bakteri dengan kode RPL-5 memilki kemampuan degrdasi terbaik pada susbtrat Malathion. Bakteri terbaik selanjutnya di uji sinergisitasnya untuk mendapatkan bakteri konsorsium terbaik yang akan digunakan dalam uji degradasi pada skala aplikasi terbatas pada air dan sedimen Rawa Pening pada konsetrasi maksimum, sebagai berikut : a) Bakteri konsorsium RPL-1 dan RPL-5 memilki kemampuan degradasi terbaik pada air Rawa Pening dengan konsentrasi Malathion b) Bakteri konsorsium RPL-5 dan TRA-5 memiliki kemampuan degradasi terbaik pada air Rawa Pening dengan konsentrasi Profenofos. Lake Rawa Pening is an ecological system which happens to a relationship the interaction between biotic factors, abiotic factors and microbial organisms, which establish a balance in it. The presence of pesticides in Rawa Pening waters led to disruption of ecological balance causing the occurrence of pollution and if it takes place in a prolonged period of time can lead to bio-accumulation at ecosystem Rawa Pening Bioremediation of pesticide Organophosphat (Profenofos and Malathion) and utilize Indigenous bacteria from Rawa Pening is an effort to restore the ecologica balance of the water due to leaching of Profenofos and Malathion. In the process of Bioremediation bacteria required elected and selected therefore required a screening process involves the isolation, identification and genetic information to get the bacteria have the ability best degradation. Screening is done by qualitative and quantitative experimental method in the laboratory. 1) Qualitative Test is done by observing the color change with Bromtymol Blue as an indicator changes color on solid zobell media enriched with Malathion and Profenofos.). 2) Quantitative tests done by the method of Gas Chromatography and Mass Spektofotmetry Spectrofotometry (GC-MS). 3) Based on the selection of bacteria through qualitative and quantitative testing are the three best bacteria with code RPL -5, TRA-5 and RPL-1. Bacteria with the code TRA-5 has the best substrate degradation abilities of Profenofos and bacteria with code RPL-5 has the best degradation on susbtrat ability of Malathion The next best in the bacterial synergistic test to get the best Consortium bacteria to be used in a test of degradation on a scale of application is limited to water and sediment Rawa Pening on maximum concentrations, as follows : 1) Bacterial Consortium with the code RPL-5 and RPL-1 has the best on subastrat degradation ability test of water from Rawa Pening with the addition of concentrations of Malathion 2) Bacterial Consortium with the code RPL-5 and TRA-5 has the best on subastrat degradation ability test of water from Rawa Pening with the addition of concentrations of Profenofos xlii Identification of bacteria selected and most superior in Malathion degrades and Profenofos done in molecular biology shows the following results: 1) The maximum identity similarity levels of bacteria RPL-1 is 87% and based on the analysis of the phylogenetic tree, the bacteria isolates RPL-1 had the closest kinship with Oceanobacillus iheyenis 2) The maximum identity similarity levels of bacteria RPL-5 is 99% and based on the analysis of the phylogenetic tree, the bacteria isolates RPL-5 has the closest kinship with the bacteria Exiquobacterium profundum 3) The maximum identity similarity levels of TRA-5 is 98% based on the phylogenetic tree analysis of bacterial isolates, TRA-5 has a very close kinship with Bacillus firmus Keywords: Pollution Of Pesticide, Malathion, Profenofos, Bioremediation, Molecular Biology Degradation, Bioremediatio

The Prediction of Air Quality Status

Construction of the National Railway Station Cross Station Kedundang - New Yogyakarta Airport Station is an accelerated development program in supporting the economy of the special region of Yogyakarta. Construction of the railroad as a consequence of infrastructure development that enables potential impacts on the surrounding environment. This study is a predictive study of air quality that might occur after operational construction of a fire pathway with Nitrogen Dioxide (NO2) with the Gas Sampler-Spectrophotometer-Saltzman Method, Carbon Monoxide (CO) with the gas sampler-NDIR analyzer method and dust particles with the dust sample, Hi-Vol gravimetric method. The data obtained is then converted into modeling using Caline 4 software, so that air quality prediction is obtained at the time of operation. Air quality category predictions use the standard air pollution index standard. The results of the analysis of the air quality parameters show a good category, only on the CO2 parameters that address high concentrations. however, based on CO2 conversion using the value of the Air Pollution Standard Index is predicted to remain in the "Good" category at the time of project operation. Therefore an air quality study is needed in the railroad development plan through an analysis study of environmental impacts, so that the management and monitoring of air quality can be carried out properly so as to cause disruption to the environment

Management of Medical Record Unit Services for Covid-19 Patients in Type C Hospitals: A Study of Legal Aspects.

Background: The impact of the pandemic on medical record health service procedures has induced significant changes. The duplication of Covid-19 patient numbers in the patient medical record unit is an important concern. This study aims to analyze the legal aspects of managing medical record services during the Covid-19 pandemic in hospitals. Methods: This type of research is descriptive and quantitative with a cross-sectional approach. The key informants are 15 registration and medical record unit officers, using online interview techniques, employed in three hospitals in Semarang with a focus on group discussions. The object of research includes the management of medical records and service standards for Covid-19 patients as regulations in the medical record unit of the Semarang city general hospital. Results: Policies and standards for medical record services for Covid-19 patients, have not been adjusted to the provisions based on Circular Letter Number HM.01.01/001/III/2020 concerning procedures for work in situations of the Covid-19 outbreak. The medical record service for Covid-19 patients still applies paper-based or semi-electronic medical records so that errors can occur. This can cause legal problems for hospitals; therefore, medical record service standards must meet legal aspects as legal evidence that can protect the interests of various parties. Conclusions: It is necessary to manage a medical resume form design that is better, complete, electronical, and in accordance with legal aspects

Toxicity Test for the Extract of Symbiont Bacteria Bacillus sp. as Anti-bacteria

Increased antibiotic resistance spurs exploration of bioactive compounds as new antibiotic alternatives. Bacillus sp. is a symbiont bacterium which is a marine microorganism that has the potential to produce new bioactive compounds that can be developed as new antibiotics. This research is an experimental study aimed at identifying bioactive compounds by thin layer chromatography methods and testing the activity of bioactive compounds by probit analysis EPA probit analysis program version 1.5. in Artemia salina Leach. Bioactive compounds identified were compounded from the alkaloid group with the category of highly toxic several-irritating base on the EPA toxicity category and the highly hazardous base on The WHO toxicity category based on the environmental protection agency probit analysis program used for calculating LC / EC value of LC 50 = 169,520 g / l. Symbiont Bacillus sp. produce secondary metabolites in the form of bioactive which have the ability as anti-bacteria. As a new antibiotic alternative to overcome resistance, especially in methicillin-resistant bacteri